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cell proliferation dye eflour e450 ebioscience  (Thermo Fisher)


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    Thermo Fisher cell proliferation dye eflour e450 ebioscience
    Cell Proliferation Dye Eflour E450 Ebioscience, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cell+proliferation+dye+eflour+e450+ebioscience/DEXTRAN/pm35705052-253-197-202
    Average 99 stars, based on 1 article reviews
    cell proliferation dye eflour e450 ebioscience - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Recombinant:

    Article Title: Vps33B controls Treg cell suppressive function through inhibiting lysosomal nutrient sensing complex-mediated mTORC1 activation.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies VPS33B Santa Cruz Cat# sc-398322 LAMP1 Abcam Cat# ab24170 RRID: AB_775978 LAMP1 Santa Cruz Cat# sc-17768 RRID: AB_626851 Mtor CST Cat# 2983T RRID: AB_2105622 P-P70 S6K(T389) CST Cat# 9234T RRID: AB_2269803 RHEB Abclonal Cat# A3702 RRID: AB_2863124 RAB7 Abclonal Cat# A12308 RRID: AB_2861652 ACTIN Santa Cruz Cat# sc-69879 RRID: AB_1119529 HSP90 Zen Bioscience Cat# 251357 EGFR Abclonal Cat# A11577 RRID: AB_2758607 RRADG Abclonal Cat# A9979 RRID: AB_2772112 SLC38A9 Sigma Cat# HPA043785 RRID: AB_10961859 LAT1 CST Cat# 9166T RRID: AB_2283298 ASCT2 CST Cat#5345 RRID: AB_10621427 LAMTOR4 CST Cat# 13140 RRID: AB_2798129 CD98 GeneTex Cat# GTX54716 RRID: AB_2887876 Tubulin Abclonal Cat# AC008 RRID: AB_2773006 CD32/16 BD Biosciences Cat# 553142 RRID: AB_394657 CD4-FITC BD Biosciences Cat# 557307 RRID: AB_396633 CD8- APCCY7 BD Biosciences Cat# 557654 RRID: AB_396769 CD44-PE BD Biosciences Cat# 553134 RRID: AB_394649 CD62L-APC BD Biosciences Cat# 553152 RRID: AB_398533 Foxp3-APC BD Biosciences Cat# 560401 RRID: AB_1645201 IFN-g-APC BD Biosciences Cat# 554413 RRID: AB_398551 IL-17-PE BD Biosciences Cat# 559502 RRID: AB_397256 (Continued on next page) e1 Cell Reports 39, 110943, June 14, 2022 .. REAGENT or RESOURCE SOURCE IDENTIFIER CD25-PECY7 BD Biosciences Cat# 552880 RRID: AB_394509 CD45.2-PB BD Biosciences Cat# 560697 RRID: AB_1727495 CD8-PE BD Biosciences Cat# 553032 RRID: AB_394570 CD4-BV421 BD Biosciences Cat# 562891 RRID: AB_2737870 GITR-PECY7 Invitrogen Cat# 25-5874-80 RRID: AB_10544396 CTLA-4-APC Invitrogen Cat# 17-1522-80 RRID: AB_2016631 Ki67-PE Invitrogen Cat# 12-5698-82 RRID: AB_11150954 CD45.1-percp-cy5.5 Invitrogen Cat# 45-0453-82 RRID: AB_1107003 Foxp3-eFluorTM 450 Invitrogen Cat# 48-5773-82 RRID: AB_1518812 CD45.2-APC-eFluorTM 780 Invitrogen Cat# 47-0454-82 RRID: AB_1272175 CD45RB-PE Biolegend Cat# 103308 RRID: AB_313015 CD45-percp Biolegend Cat# 103130 RRID: AB_313015 IL-17-percp-cy5.5 Biolegend Cat# 506919 RRID: AB_961385 hCD45-FITC Biolegend Cat# 304006 RRID: AB_314394 hCD4-APC Biolegend Cat# 317416 RRID: AB_571945 hCD25-PE Biolegend Cat# 302606 RRID: AB_314276 hCD8-PE-CY7 BD Biosciences Cat# 557746 RRID: AB_396852 Alexa Fluor 488 goat anti-Rabbit Invitrogen Cat# A21245 RRID: AB_2535813 Alexa Fluor 555 donkey anti-Mouse Invitrogen Cat# A31570 RRID: AB_2536180 Anti-mouse IgG HRP-linked Antibody CST Cat# 7076 RRID: AB_330924 Anti-rabbit IgG HRP-linked Antibody CST Cat# 7074 RRID: AB_2099233 Chemicals, peptides, and recombinant proteins Purified Hamster Anti-Mouse CD3e BD Biosciences Cat# 553058 Purified NA/LE Hamster Anti-Mouse CD28 BD Biosciences Cat# 553294 ImmunoCultTM Mouse Treg Differentiation Supplement STEMCELL Cat# 10957 TrizolTM Reagent Ambion Cat# 15596026 ECL WB substrate Tanon Cat# 180-5001 CFSE Biolegend Cat# 79898 Cell proliferation dye eFlour E450 eBioscience Cat# 65-0842 Dextran, Alexa FluorTM 647 Invitrogen Cat# D22914 (Continued on next page) Cell Reports 39, 110943, June 14, 2022 e2 Article ll OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER LysoTracker Red DND-99 Invitrogen Cat# L7528 Cell-Tak (Corning) CORNING Cat# 354240 DNase I STEMCELL Cat# 9003-98-9 SYBR Green Yeasen Cat# 11201ES03 Phosphatase inhibitors keygen biotech Cat# KGP602 DCA Selleck Cat# S8615 Agilent Seahorse XF glycolysis stress test kit Agilent Cat# 103020-100 Agilent Seahorse XF Cell Mito Stress test kit Agilent Cat# 103015-100 RPMI without amino acid Coolaber science Cat# CM0011 Triton X Beyotime Cat# ST795 Bovine Serum Albumin Yeasen Cat# 36101ES60 Percoll GE-healthcare Cat# 17-5446-02 Ficoll GE-healthcare Cat# 26873-85-8 Optiprep Sigma Cat# 0594145v Dynasore MCE Cat# HY-15304 Collagenase IV Sigma Cat# 403a Collagenase D Sigma Cat# C5138 FBS GIBCO Cat# 12483020 Penicillin and streptomycin GIBCO Cat# 10378016 b-mercaptoethanol Sigma Cat# 444203 L-Glutamine STEMCELL Cat# 07100 HEPES Buffer Solution STEMCELL Cat# 07200 Sodium Pyruvate ThermoFisher Cat# 11360070 MEM Non-Essential Amino Acid Solution STEMCELL Cat# 07600 Polybrene Sigma Cat# TR-1003-G Recombinant human IL-2 Peprotech Cat# 200-02 Fxiable Viability Stain510 BD Biosciences Cat# 564406 Fxiable Viability Stain700 BD Biosciences Cat# 564997 Critical commercial assays Hifair II 1st Strand cDNA Sythesis Kit (gDNA digester plus) Yeasen Cat# 11121 PVDF membrane Immobilon-P Cat# IPVH00010 Stimulation cocktail Invitrogen Cat# 00-4975-93 eBioscienceTM Transcription Factor Fixation/ Permeabilization Concentrate and Diluent Invitrogen Cat# 00-5521-00 /00-8333-56 Agilent XF96 Cell Culture Microplates Agilent Cat# 101085-004 Seahorse XF Glycolysis Stress Test Kit Agilent Cat# 103020-100 Seahorse XF Cell Mito Stress Test Kit Agilent Cat# 103010-100 EasySepTM Mouse Naive CD4+ T cell Isolation Kit STEMCELL Cat# 19765 EasySepTM Mouse CD4+CD25+ Regulatory T cell Isolation Kit II STEMCELL Cat# 18783 Mouse T cell Nucleofector Kit Lonza Cat# VPA-1006 Human T Cells Nucleofector Kit Lonza Cat# VPA-1002 Lysosome isolation kit sigma Cat# LYSISO1 Experimental models: Cell lines Cell line: 293T ATCC Cat# CRL-3216 Cell line: Jurkat cell line ATCC Cat# TIB-152 Experimental models: Organisms/strains Mouse: Vps33Bf/f (Gu et al., 2016) N/A (Continued on next page) e3 Cell Reports 39, 110943, June 14, 2022 Article ll OPEN ACCESS

    Purification:

    Article Title: Vps33B controls Treg cell suppressive function through inhibiting lysosomal nutrient sensing complex-mediated mTORC1 activation.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies VPS33B Santa Cruz Cat# sc-398322 LAMP1 Abcam Cat# ab24170 RRID: AB_775978 LAMP1 Santa Cruz Cat# sc-17768 RRID: AB_626851 Mtor CST Cat# 2983T RRID: AB_2105622 P-P70 S6K(T389) CST Cat# 9234T RRID: AB_2269803 RHEB Abclonal Cat# A3702 RRID: AB_2863124 RAB7 Abclonal Cat# A12308 RRID: AB_2861652 ACTIN Santa Cruz Cat# sc-69879 RRID: AB_1119529 HSP90 Zen Bioscience Cat# 251357 EGFR Abclonal Cat# A11577 RRID: AB_2758607 RRADG Abclonal Cat# A9979 RRID: AB_2772112 SLC38A9 Sigma Cat# HPA043785 RRID: AB_10961859 LAT1 CST Cat# 9166T RRID: AB_2283298 ASCT2 CST Cat#5345 RRID: AB_10621427 LAMTOR4 CST Cat# 13140 RRID: AB_2798129 CD98 GeneTex Cat# GTX54716 RRID: AB_2887876 Tubulin Abclonal Cat# AC008 RRID: AB_2773006 CD32/16 BD Biosciences Cat# 553142 RRID: AB_394657 CD4-FITC BD Biosciences Cat# 557307 RRID: AB_396633 CD8- APCCY7 BD Biosciences Cat# 557654 RRID: AB_396769 CD44-PE BD Biosciences Cat# 553134 RRID: AB_394649 CD62L-APC BD Biosciences Cat# 553152 RRID: AB_398533 Foxp3-APC BD Biosciences Cat# 560401 RRID: AB_1645201 IFN-g-APC BD Biosciences Cat# 554413 RRID: AB_398551 IL-17-PE BD Biosciences Cat# 559502 RRID: AB_397256 (Continued on next page) e1 Cell Reports 39, 110943, June 14, 2022 .. REAGENT or RESOURCE SOURCE IDENTIFIER CD25-PECY7 BD Biosciences Cat# 552880 RRID: AB_394509 CD45.2-PB BD Biosciences Cat# 560697 RRID: AB_1727495 CD8-PE BD Biosciences Cat# 553032 RRID: AB_394570 CD4-BV421 BD Biosciences Cat# 562891 RRID: AB_2737870 GITR-PECY7 Invitrogen Cat# 25-5874-80 RRID: AB_10544396 CTLA-4-APC Invitrogen Cat# 17-1522-80 RRID: AB_2016631 Ki67-PE Invitrogen Cat# 12-5698-82 RRID: AB_11150954 CD45.1-percp-cy5.5 Invitrogen Cat# 45-0453-82 RRID: AB_1107003 Foxp3-eFluorTM 450 Invitrogen Cat# 48-5773-82 RRID: AB_1518812 CD45.2-APC-eFluorTM 780 Invitrogen Cat# 47-0454-82 RRID: AB_1272175 CD45RB-PE Biolegend Cat# 103308 RRID: AB_313015 CD45-percp Biolegend Cat# 103130 RRID: AB_313015 IL-17-percp-cy5.5 Biolegend Cat# 506919 RRID: AB_961385 hCD45-FITC Biolegend Cat# 304006 RRID: AB_314394 hCD4-APC Biolegend Cat# 317416 RRID: AB_571945 hCD25-PE Biolegend Cat# 302606 RRID: AB_314276 hCD8-PE-CY7 BD Biosciences Cat# 557746 RRID: AB_396852 Alexa Fluor 488 goat anti-Rabbit Invitrogen Cat# A21245 RRID: AB_2535813 Alexa Fluor 555 donkey anti-Mouse Invitrogen Cat# A31570 RRID: AB_2536180 Anti-mouse IgG HRP-linked Antibody CST Cat# 7076 RRID: AB_330924 Anti-rabbit IgG HRP-linked Antibody CST Cat# 7074 RRID: AB_2099233 Chemicals, peptides, and recombinant proteins Purified Hamster Anti-Mouse CD3e BD Biosciences Cat# 553058 Purified NA/LE Hamster Anti-Mouse CD28 BD Biosciences Cat# 553294 ImmunoCultTM Mouse Treg Differentiation Supplement STEMCELL Cat# 10957 TrizolTM Reagent Ambion Cat# 15596026 ECL WB substrate Tanon Cat# 180-5001 CFSE Biolegend Cat# 79898 Cell proliferation dye eFlour E450 eBioscience Cat# 65-0842 Dextran, Alexa FluorTM 647 Invitrogen Cat# D22914 (Continued on next page) Cell Reports 39, 110943, June 14, 2022 e2 Article ll OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER LysoTracker Red DND-99 Invitrogen Cat# L7528 Cell-Tak (Corning) CORNING Cat# 354240 DNase I STEMCELL Cat# 9003-98-9 SYBR Green Yeasen Cat# 11201ES03 Phosphatase inhibitors keygen biotech Cat# KGP602 DCA Selleck Cat# S8615 Agilent Seahorse XF glycolysis stress test kit Agilent Cat# 103020-100 Agilent Seahorse XF Cell Mito Stress test kit Agilent Cat# 103015-100 RPMI without amino acid Coolaber science Cat# CM0011 Triton X Beyotime Cat# ST795 Bovine Serum Albumin Yeasen Cat# 36101ES60 Percoll GE-healthcare Cat# 17-5446-02 Ficoll GE-healthcare Cat# 26873-85-8 Optiprep Sigma Cat# 0594145v Dynasore MCE Cat# HY-15304 Collagenase IV Sigma Cat# 403a Collagenase D Sigma Cat# C5138 FBS GIBCO Cat# 12483020 Penicillin and streptomycin GIBCO Cat# 10378016 b-mercaptoethanol Sigma Cat# 444203 L-Glutamine STEMCELL Cat# 07100 HEPES Buffer Solution STEMCELL Cat# 07200 Sodium Pyruvate ThermoFisher Cat# 11360070 MEM Non-Essential Amino Acid Solution STEMCELL Cat# 07600 Polybrene Sigma Cat# TR-1003-G Recombinant human IL-2 Peprotech Cat# 200-02 Fxiable Viability Stain510 BD Biosciences Cat# 564406 Fxiable Viability Stain700 BD Biosciences Cat# 564997 Critical commercial assays Hifair II 1st Strand cDNA Sythesis Kit (gDNA digester plus) Yeasen Cat# 11121 PVDF membrane Immobilon-P Cat# IPVH00010 Stimulation cocktail Invitrogen Cat# 00-4975-93 eBioscienceTM Transcription Factor Fixation/ Permeabilization Concentrate and Diluent Invitrogen Cat# 00-5521-00 /00-8333-56 Agilent XF96 Cell Culture Microplates Agilent Cat# 101085-004 Seahorse XF Glycolysis Stress Test Kit Agilent Cat# 103020-100 Seahorse XF Cell Mito Stress Test Kit Agilent Cat# 103010-100 EasySepTM Mouse Naive CD4+ T cell Isolation Kit STEMCELL Cat# 19765 EasySepTM Mouse CD4+CD25+ Regulatory T cell Isolation Kit II STEMCELL Cat# 18783 Mouse T cell Nucleofector Kit Lonza Cat# VPA-1006 Human T Cells Nucleofector Kit Lonza Cat# VPA-1002 Lysosome isolation kit sigma Cat# LYSISO1 Experimental models: Cell lines Cell line: 293T ATCC Cat# CRL-3216 Cell line: Jurkat cell line ATCC Cat# TIB-152 Experimental models: Organisms/strains Mouse: Vps33Bf/f (Gu et al., 2016) N/A (Continued on next page) e3 Cell Reports 39, 110943, June 14, 2022 Article ll OPEN ACCESS

    Western Blot:

    Article Title: Vps33B controls Treg cell suppressive function through inhibiting lysosomal nutrient sensing complex-mediated mTORC1 activation.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies VPS33B Santa Cruz Cat# sc-398322 LAMP1 Abcam Cat# ab24170 RRID: AB_775978 LAMP1 Santa Cruz Cat# sc-17768 RRID: AB_626851 Mtor CST Cat# 2983T RRID: AB_2105622 P-P70 S6K(T389) CST Cat# 9234T RRID: AB_2269803 RHEB Abclonal Cat# A3702 RRID: AB_2863124 RAB7 Abclonal Cat# A12308 RRID: AB_2861652 ACTIN Santa Cruz Cat# sc-69879 RRID: AB_1119529 HSP90 Zen Bioscience Cat# 251357 EGFR Abclonal Cat# A11577 RRID: AB_2758607 RRADG Abclonal Cat# A9979 RRID: AB_2772112 SLC38A9 Sigma Cat# HPA043785 RRID: AB_10961859 LAT1 CST Cat# 9166T RRID: AB_2283298 ASCT2 CST Cat#5345 RRID: AB_10621427 LAMTOR4 CST Cat# 13140 RRID: AB_2798129 CD98 GeneTex Cat# GTX54716 RRID: AB_2887876 Tubulin Abclonal Cat# AC008 RRID: AB_2773006 CD32/16 BD Biosciences Cat# 553142 RRID: AB_394657 CD4-FITC BD Biosciences Cat# 557307 RRID: AB_396633 CD8- APCCY7 BD Biosciences Cat# 557654 RRID: AB_396769 CD44-PE BD Biosciences Cat# 553134 RRID: AB_394649 CD62L-APC BD Biosciences Cat# 553152 RRID: AB_398533 Foxp3-APC BD Biosciences Cat# 560401 RRID: AB_1645201 IFN-g-APC BD Biosciences Cat# 554413 RRID: AB_398551 IL-17-PE BD Biosciences Cat# 559502 RRID: AB_397256 (Continued on next page) e1 Cell Reports 39, 110943, June 14, 2022 .. REAGENT or RESOURCE SOURCE IDENTIFIER CD25-PECY7 BD Biosciences Cat# 552880 RRID: AB_394509 CD45.2-PB BD Biosciences Cat# 560697 RRID: AB_1727495 CD8-PE BD Biosciences Cat# 553032 RRID: AB_394570 CD4-BV421 BD Biosciences Cat# 562891 RRID: AB_2737870 GITR-PECY7 Invitrogen Cat# 25-5874-80 RRID: AB_10544396 CTLA-4-APC Invitrogen Cat# 17-1522-80 RRID: AB_2016631 Ki67-PE Invitrogen Cat# 12-5698-82 RRID: AB_11150954 CD45.1-percp-cy5.5 Invitrogen Cat# 45-0453-82 RRID: AB_1107003 Foxp3-eFluorTM 450 Invitrogen Cat# 48-5773-82 RRID: AB_1518812 CD45.2-APC-eFluorTM 780 Invitrogen Cat# 47-0454-82 RRID: AB_1272175 CD45RB-PE Biolegend Cat# 103308 RRID: AB_313015 CD45-percp Biolegend Cat# 103130 RRID: AB_313015 IL-17-percp-cy5.5 Biolegend Cat# 506919 RRID: AB_961385 hCD45-FITC Biolegend Cat# 304006 RRID: AB_314394 hCD4-APC Biolegend Cat# 317416 RRID: AB_571945 hCD25-PE Biolegend Cat# 302606 RRID: AB_314276 hCD8-PE-CY7 BD Biosciences Cat# 557746 RRID: AB_396852 Alexa Fluor 488 goat anti-Rabbit Invitrogen Cat# A21245 RRID: AB_2535813 Alexa Fluor 555 donkey anti-Mouse Invitrogen Cat# A31570 RRID: AB_2536180 Anti-mouse IgG HRP-linked Antibody CST Cat# 7076 RRID: AB_330924 Anti-rabbit IgG HRP-linked Antibody CST Cat# 7074 RRID: AB_2099233 Chemicals, peptides, and recombinant proteins Purified Hamster Anti-Mouse CD3e BD Biosciences Cat# 553058 Purified NA/LE Hamster Anti-Mouse CD28 BD Biosciences Cat# 553294 ImmunoCultTM Mouse Treg Differentiation Supplement STEMCELL Cat# 10957 TrizolTM Reagent Ambion Cat# 15596026 ECL WB substrate Tanon Cat# 180-5001 CFSE Biolegend Cat# 79898 Cell proliferation dye eFlour E450 eBioscience Cat# 65-0842 Dextran, Alexa FluorTM 647 Invitrogen Cat# D22914 (Continued on next page) Cell Reports 39, 110943, June 14, 2022 e2 Article ll OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER LysoTracker Red DND-99 Invitrogen Cat# L7528 Cell-Tak (Corning) CORNING Cat# 354240 DNase I STEMCELL Cat# 9003-98-9 SYBR Green Yeasen Cat# 11201ES03 Phosphatase inhibitors keygen biotech Cat# KGP602 DCA Selleck Cat# S8615 Agilent Seahorse XF glycolysis stress test kit Agilent Cat# 103020-100 Agilent Seahorse XF Cell Mito Stress test kit Agilent Cat# 103015-100 RPMI without amino acid Coolaber science Cat# CM0011 Triton X Beyotime Cat# ST795 Bovine Serum Albumin Yeasen Cat# 36101ES60 Percoll GE-healthcare Cat# 17-5446-02 Ficoll GE-healthcare Cat# 26873-85-8 Optiprep Sigma Cat# 0594145v Dynasore MCE Cat# HY-15304 Collagenase IV Sigma Cat# 403a Collagenase D Sigma Cat# C5138 FBS GIBCO Cat# 12483020 Penicillin and streptomycin GIBCO Cat# 10378016 b-mercaptoethanol Sigma Cat# 444203 L-Glutamine STEMCELL Cat# 07100 HEPES Buffer Solution STEMCELL Cat# 07200 Sodium Pyruvate ThermoFisher Cat# 11360070 MEM Non-Essential Amino Acid Solution STEMCELL Cat# 07600 Polybrene Sigma Cat# TR-1003-G Recombinant human IL-2 Peprotech Cat# 200-02 Fxiable Viability Stain510 BD Biosciences Cat# 564406 Fxiable Viability Stain700 BD Biosciences Cat# 564997 Critical commercial assays Hifair II 1st Strand cDNA Sythesis Kit (gDNA digester plus) Yeasen Cat# 11121 PVDF membrane Immobilon-P Cat# IPVH00010 Stimulation cocktail Invitrogen Cat# 00-4975-93 eBioscienceTM Transcription Factor Fixation/ Permeabilization Concentrate and Diluent Invitrogen Cat# 00-5521-00 /00-8333-56 Agilent XF96 Cell Culture Microplates Agilent Cat# 101085-004 Seahorse XF Glycolysis Stress Test Kit Agilent Cat# 103020-100 Seahorse XF Cell Mito Stress Test Kit Agilent Cat# 103010-100 EasySepTM Mouse Naive CD4+ T cell Isolation Kit STEMCELL Cat# 19765 EasySepTM Mouse CD4+CD25+ Regulatory T cell Isolation Kit II STEMCELL Cat# 18783 Mouse T cell Nucleofector Kit Lonza Cat# VPA-1006 Human T Cells Nucleofector Kit Lonza Cat# VPA-1002 Lysosome isolation kit sigma Cat# LYSISO1 Experimental models: Cell lines Cell line: 293T ATCC Cat# CRL-3216 Cell line: Jurkat cell line ATCC Cat# TIB-152 Experimental models: Organisms/strains Mouse: Vps33Bf/f (Gu et al., 2016) N/A (Continued on next page) e3 Cell Reports 39, 110943, June 14, 2022 Article ll OPEN ACCESS



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